Do Not Need this info for Spring (but still copy these lines to your Target page for now)
*GC% Content for gene: output
*GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): (link to DNA Works outputtext file-that should be saved in your Google Docs folder after you did the primer design protocol) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):
Target (protein/gene name): AMP deaminase, putative
NCBI #: S59996
EC #: 3.5.4.6
Protein ID (NP or XP #) or Wolbachia#: MAL13P1.146
Organism (including strain): malaria parasite Plasmodium falciparum
Etiologic Risk Group (see link below): ??
Disease Information(sort of like the Intro to your MiniResearch Writeup):
Plasmodium falciparum is a protozoan parasite that causes malaria. P. falciparum is transmitted by mosquitoes.
Link to TDR Targets page (if present): http://tdrtargets.org/targets/view?gene_id=54
Link to Gene Database page NCBI: https://www.ncbi.nlm.nih.gov/protein/S59996
Essentiality of this protein:
Gene/Ortholog: Tb11.02.1340 (OG4_10329); Phenotype: significant loss of fitness in bloodstream forms (6 days); Source study: alsford
Is it a monomer or multimer as biological unit: ??
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
The druggable index for AMP deaminase, putative on 0-1 scale is a 0.8. This target has been proved to be druggable in a number of other organisms.
https://www.ncbi.nlm.nih.gov/pubmed/10780906
https://www.ncbi.nlm.nih.gov/pubmed/10780906
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/enzyme.php?ecno=3.5.4.6
Enzyme Assay information:
http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/assay-library/ec-number-iii.html
Structure (PDB or Homology model)
No structure available in the PDB. No known Homology model.
PDB #:
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
Length of your protein in Amino Acids: 672 amino acids.
Molecular Weight of your protein in kiloDaltons: 79309 DA
Molar Extinction coefficient of your protein at 280 nm wavelength: 59750 mol^-1cm-1
TMpred graph Image: http://embnet.vital-it.ch/cgi-bin/TMPRED_form_parser
*CDS Gene Sequence (paste as text only):
Do Not Need this info for Spring (but still copy these lines to your Target page for now)
*GC% Content for gene:
output
*GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
(link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):